Simple and Efficient Methods for Enrichment and Isolation of Endonuclease Modified Cells.

May 1, 2014
Authors: Moriarity, et al.
B-MoGen Authors: Branden S. Moriarity & David A. Largaespada
Publication: PLOSone. 9(5): e96114. doi: 10.1371/journal.pone.0096114
Published Date: May 2014
Describes new methodology for reducing time spent single-cell cloning a cell population with a knockout deletion.
The authors developed two methods to select nuclease-edited cells. Co-transposition (now known as GEEK) and co-targeting utilize induced phenotypic changes that allow for enrichment of gene edited cells. Unlike FACS and reporter-based enrichment strategies, the methods presented are simple, efficient, allow for isolation of single-cell derived clones. These methods expedite the generation of knockout cells lines and have the potential to improve the generation of therapeutic cells.