Modeling Hepatitis B Virus X-Induced Hepatocellular Carcinoma in Mice with the Sleeping Beauty Transposon System.

December 1, 2010
Authors: Keng, et al.
B-MoGen Authors: David A Largaespada
Publication: Hepatology. 53(3): 781-790.
Published Date: December 2010
Describes system that can be used as a model for studying the various genetic contributions of hepatitis B virus (HBV) to liver hyperplasia and hepatocellular carcinoma (HCC).
Unique regulatory component gene X of HBV (HBx) has been shown to induce cell proliferation and proapoptotic and stress response pathways, though the oncogenic mechanisms of HBx are divisive. Because there is no good animal model for HBx oncogenesis, the authors used the Sleeping Beauty transposon to stably deliver the HBx transgene to mice. As a result, the authors were able to mimic HBx expression after HBV infection and the repopulation of HBV-infected hepatocytes in the liver.