A Sleeping Beauty forward genetic screen identifies new genes and pathways driving osteosarcoma development and metastasis.

May 1, 2015
Authors: Moriarity, et al.
B-MoGen Authors: Branden S. Moriarity and David A. Largaespada
Publication: Nature Genetics. 47(6): 615-624.
Published Date: May 2015
Demonstrates how Sleeping Beauty transposon system allows for next-generation genetic screens that identify disease-specific genes and pathways implicated in metastasis.
A characteristic of osteosarcoma is significant genomic instability, which makes identification of disease-specific driver genes challenging. Using a conditional Sleeping Beauty transposon mutagenesis system, authors identified 232 osteosarcoma-related genes associated with recurrent transposon insertion sites. This study validated previously implicated genes in osteosarcoma and identified new genes that are enriched in cancer-associated signaling pathways. These genes, which are likely driving pathways in human osteosarcoma, represent potential therapeutic targets.