Multiple Cloning Site (MCS) ready SBT vectors are an affordable way to generate your own custom Sleeping Beauty transposon vectors. Our MCS region is compatible with several restriction enzyme sites for ease of use and is followed by a stable reporter for easy selection and use in downstream experiments. Clone and ligate your desired gene into an MCS ready SBT vector. Co-transfection with an SB11 transposase expression vector to put your gene of interest into your cells.
- Vector backbone contains two sequencing primer sites and two PvuII restriction enzyme cut sites.
- Cytomegalovirus Promoter boasts high levels of gene expression in most mammalian cells, especially in neurons and embryonic stem cells.
- The multiple cloning site (MCS) allows for the insertion of desired genes into our Sleeping Beauty transposon vectors. We follow our MCS with an IRES element to ensure consistent expression of both MCS inserted cargo and reporter genes. MCS site is prepped for digest with EcoRI and EcoRV restriction enzymes.
- Neomycin resistance gene enables drug selection with G418 of transposon containing cells.